Fig. 3.
Fibrinogen Longmont is protected by both GPRP peptide and calcium ions against further digestion by plasmin.
Normal fibrinogen and fibrinogen Longmont (0.2 mg/m) were digested with 10 μg/mL plasmin in the presence of 5 mM EDTA, 5mM CaCl2, or 2 mM GPRP peptide. The plasmin digests were analyzed on 7.5% SDS-PAGE in nonreduced conditions. (A) The gel was stained with Coomassie brilliant blue. (B) A Western blot of the plasmin digests in the presence of 5 mM EDTA or 5mM CaCl2 using a polyclonal antibody to human fibrinogen.
