Static and dynamic light scattering: thrombin treatment of fibrinogen Longmont leads to protofibril formation.

Thrombin (0.001 NIH U/mL) was added to purified Fr II of normal fibrinogen (closed circles) or of fibrinogen Longmont (closed triangles), and the intensity of the light scattered was monitored for 30 minutes. Representative curve of the intensity of light scattered (I/I₀) over time (A); representative curve of the change in translational diffusion coefficient (D/D₀) and the equivalent of the growing protofibrils size (second y-axis) over time (B). The dashed line indicates the formation of protofibrils.