RT-PCR analysis of hPF4 versus mPF4.

(A) Ethidium bromide analysis as in Figure 2A, but for PF4. (B) Relationship between the copy number of the transgene and the platelet level of message of human versus mouse PF4 was determined for the PF4-expression lines λ-PBP/PF4 and PF4-Only. The determination of the relative quantity of hPF4 to mPF4 message was as described in Figure 2B. ■, PF4-Only; ♦,λ-PBP/PF4. Data are shown for average ± 1 SD. Experiments were repeated 3 separate times.