Immunodetection of hPF4 in the transgenic mice platelets.

(A) SDS-PAGE gels of equal quantities of platelet protein were immunoblotted with an anti-hPF4 monoclonal antibody RTO that does not recognize mPF4 proteins. Platelet proteins from the transgenic linesPF4-Only and λ-PBP/PF4 were studied. Control lanes include wild-type mice and 4 different normal human volunteers (a through d). (B) The relative expression of hPF4 in the transgenic mice to the average value in the 4 human samples per milligram total platelet protein was determined and compared to its copy number. The dotted line is that average human platelet hPF4 value. ■PF4-Only; ♦, λ-PBP/PF4. Data are shown for average ± 1 SD.