Fig. 7.
Fig. 7. C/EBPε is critical in myc-induced differentiation block in 32D/myc cells. / (A) Expression of C/EBPε, C/EBPα, and MPO in 32D/myc cells. Cells maintained in IL-3 were washed and transferred to medium containing 50 ng/mL G-CSF. The cells were harvested at the times indicated and subjected to Northern blot analysis. (B) Ectopic expression of C/EBPε induces differentiation in 32D/myc cells. C/EBPε expression vector (20 μg) with a cistronic zeomycin-resistance marker (pcDNA3Zeo-C/EBPε) was electroporated into 32D/myc cells, and transfected cells were selected with 750 μg/mL zeomycin for 6 days. The empty vector was used as a negative control (i indicates negative control; and ii-iv, C/EBPε-transfected cells). Morphologic features of cells were visualized by using Wright-Giemsa staining (original magnification, ×400).

C/EBPε is critical in myc-induced differentiation block in 32D/myc cells.

(A) Expression of C/EBPε, C/EBPα, and MPO in 32D/myc cells. Cells maintained in IL-3 were washed and transferred to medium containing 50 ng/mL G-CSF. The cells were harvested at the times indicated and subjected to Northern blot analysis. (B) Ectopic expression of C/EBPε induces differentiation in 32D/myc cells. C/EBPε expression vector (20 μg) with a cistronic zeomycin-resistance marker (pcDNA3Zeo-C/EBPε) was electroporated into 32D/myc cells, and transfected cells were selected with 750 μg/mL zeomycin for 6 days. The empty vector was used as a negative control (i indicates negative control; and ii-iv, C/EBPε-transfected cells). Morphologic features of cells were visualized by using Wright-Giemsa staining (original magnification, ×400).

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