Production of IL-4, IL-5, and IFN-γ by T cells from tolerant, immunized, and naive CBA spleen T cells in mLC.

Spleen T cells from tolerant (▵), immunized (○), and naive (■) CBA mice were stimulated with BALB/c spleen cells treated with 25 Gy gamma irradiation (experiment ) or 50 μg/mL mitomycin C (experiment 2) in mLC. Supernatants were harvested and pooled from triplicate wells daily and assayed for cytokines as described.