CCR7⁻ CD8⁺ T cells are among CD27⁺ and CD27⁻ cells.

(A) Histogram of CD8 expression (x-axis, arbitrary units, log scale) versus cell number (y-axis) in PBMCs from one representative healthy individual. The horizontal line designates the gate for CD8^bright lymphocytes. (B) Histogram of CCR7 expression (x-axis, arbitrary units, log scale) versus cell number (y-axis) in gated CD8^bright T cells. The horizontal line designates the gate for CCR7⁻ cells (black). (C) Dot plot of CD27 expression (x-axis, arbitrary units, log scale) versus CD45RA expression (y-axis, arbitrary units, log scale) in gated CD8^bright T cells. CCR7⁻ cells are represented in black, whereas CCR7⁺ cells are represented in gray. (D) Dot plot of CD28 expression (x-axis, arbitrary units) versus CD45R0 expression (y-axis, arbitrary units) in gated CD8^bright T cells. CCR7⁻ cells are represented in black, whereas CCR7⁺ cells are represented in gray.