Flow cytometric analysis of normal, mutant, and mock-transfected cells.

Surface expression of GPIIIa, GPIIb/IIIa complex, and vitronectin receptor (VnR) in BHK cells transfected with normal GPIIb cDNA and either normal or mutant GPIIIa cDNAs. Monoclonal antibodies used were AP3 against GPIIIa, 10E5 against GPIIb/IIIa, and LM609 against vitronectin receptor. LM609 detects a complex of hamster α_v and human GPIIIa. The black histogram represents the binding of FITC-labeled secondary antibody. It can be seen that binding of all 3 antibodies is similar for WT and C655S cells, whereas no binding is detectable for Stop657, IJ-1, and mock cells. The x-axis represents fluorescence intensity, and the y-axis represents total events.