Detection of GPIIIa and GPIIb/IIIa complexes within transfected cells using immunohistochemical staining.

Transfected cells were stained with monoclonal antibodies AP5 and 10E5 against GPIIIa and GPIIb/IIIa complex, respectively. Antibody binding was determined by alkaline phosphatase–antialkaline phosphatase staining. It can be seen that GPIIIa was detected in cells containing IJ-1, Stop657, C655S, and WT, whereas GPIIb/IIIa was detected in WT and C655S but not in IJ-1 and Stop657. Original magnification, × 200-400..