Detection of GPIIIa and GPIIb/IIIa complex in lysates of transfected cells.

(A) Transfected CHO cell lysates were immunoprecipitated by using polyclonal antibodies against GPIIb and GPIIIa and then detected by immunoblotting using AP5 antibody against GPIIIa. GPIIIa of WT and C655S-containing cells migrated normally at approximately 90 kd, whereas GPIIIa of IJ-1 and Stop657 cells migrated more rapidly, consistent with a truncated protein. (B) Transfected BHK cell lysates were immunoprecipitated using 10E5 monoclonal antibody against GPIIb/GPIIIa complex and detected by immunoblotting using polyclonal antibodies against GPIIb and GPIIIa. GPIIb and GPIIIa were detected in WT and C655S cells, indicating the presence of GPIIb/IIIa complex in these cells, but not in IJ-1, Stop657, and mock cells. Numbers on the left represent molecular weight standards.