Ascorbic acid potentiates As₂O₃-mediated disruption of mitochondrial membrane potential.

Cells (2 × 10⁵) were cultured in the absence (dashed line) or the presence of As₂O₃ (2 μM; solid line), AA (100 μM; dotted), or As₂O₃+AA (bold) for 48 hours. Mitochondrial membrane potential was measured by TMRE fluorescence and FACScan analysis as described in “Materials and methods.” Cell viability (inset) was monitored by Annexin V–FITC and PI staining followed by FACScan analysis. Data are representative of 3 independent experiments per cell line.