American Society of Hematology

Fig. 9.

$Fig. 9. Plasma cells from patients with multiple myelomas are sensitive to As2O3 and ascorbic acid. / (A) BM mononuclear cells were isolated from patients with newly diagnosed MM (n = 9) and cultured in the absence or presence of As2O3 (2 μM; ░), AA (100 μM; ▨), As2O3+AA (▪), or etoposide (10 μg/mL; ■) for 48 hours, as described in “Material and methods.” Cells were then triple stained with phycoerythrin-conjugated anti-CD38, CyChrome conjugated anti-CD45, and FITC-conjugated Annexin V. Death of CD38/CD45− cells (myeloma cells) and of the CD38− population (nonmyeloma cells) was determined by FACScan analysis. Data are presented as the mean ± SE of the percentage apoptotic (Annexin V–FITC+) cells in treated wells minus percentage apoptotic cells in untreated wells. Statistical analyses were performed using a paired t test, where AA+As differs from AA and etoposide in the CD38/CD45− population (**P < .0006) and As differs from AA in the CD38− population (*P < .03). (B) BM mononuclear cells were isolated from patients with refractory MM (n = 14), treated, and analyzed as described in panel A. Statistical analyses were performed using a paired t test, where AA+As differs from As, AA, and etoposide in the CD38/CD45−population (**P < .02) and AA+As differs from AA in the CD38− population (*P < .03).$

Plasma cells from patients with multiple myelomas are sensitive to As₂O₃ and ascorbic acid.

(A) BM mononuclear cells were isolated from patients with newly diagnosed MM (n = 9) and cultured in the absence or presence of As₂O₃ (2 μM; ░), AA (100 μM; ▨), As₂O₃+AA (▪), or etoposide (10 μg/mL; ■) for 48 hours, as described in “Material and methods.” Cells were then triple stained with phycoerythrin-conjugated anti-CD38, CyChrome conjugated anti-CD45, and FITC-conjugated Annexin V. Death of CD38/CD45⁻ cells (myeloma cells) and of the CD38⁻ population (nonmyeloma cells) was determined by FACScan analysis. Data are presented as the mean ± SE of the percentage apoptotic (Annexin V–FITC+) cells in treated wells minus percentage apoptotic cells in untreated wells. Statistical analyses were performed using a paired t test, where AA+As differs from AA and etoposide in the CD38/CD45⁻ population (**P < .0006) and As differs from AA in the CD38⁻ population (*P < .03). (B) BM mononuclear cells were isolated from patients with refractory MM (n = 14), treated, and analyzed as described in panel A. Statistical analyses were performed using a paired t test, where AA+As differs from As, AA, and etoposide in the CD38/CD45⁻population (**P < .02) and AA+As differs from AA in the CD38⁻ population (*P < .03).

This Feature Is Available To Subscribers Only