Cytokine mRNA expression in Tax transgenic mouse tumors.

Total RNA (10 μg) was extracted from fresh NT (lane 1) and Tax transgenic mouse tissues (lanes 2 and 3) or from the tumor-derived F8 LGL cell line (lane 4) and used in RNase protection assays using a multiprobe cytokine system. The L32 and GAPDH probes were used to demonstrate equivalent RNA levels in each sample. A total of 4 primary Tax-induced tumors and 5 Tax spleens were analyzed, and a representative gel is shown.