Sodium salicylate inhibits cytokine mRNA expression in mouse splenocytes.

Total RNA (10 μg) was extracted from NT (lanes 7-10) and Tax transgenic (lanes 1-6) mouse spleens following treatment with sodium salicylate (SA, lanes 2-5 and 8-9) or neutralizing cytokine antibodies (lanes 6 and 10) for 16 hours. NT splenocytes were also stimulated with 500 U/mL IL-2 plus 10 μg/mL PHA. Neutralizing antibodies included monoclonal antibodies to IL-6 and IL-10 (1 μg/mL), polyclonal antibodies to IFN-γ (10 μg/mL), and monoclonal antibodies to the IL-2Rβ chain (10 μg/mL). Irrelevant GST monoclonal antibodies (10 μg/mL) were used as negative controls (lanes 1 and 7). RNase protection assays were performed as described in Figure 1. A total of 3 spleens from NT mice and 3 spleens from Tax transgenic mice were tested, and a representative gel is shown. Lanes 2 and 3 and lanes 4 and 5 are each duplicate samples of the same cultured splenocytes.