Gp120-induced MMP-9 secretion in C6 glioma cells.

C6 glioma cells were treated for 18 hours with or without (unstimulated) the indicated concentration of gp120/HXB2, gp120/JRFL, SDF-1α, MIP-1 β, IL-1 α, TNF-α, or lipopolysaccharide. Supernatants were collected, lyophilized, and assayed for their gelatinase content by zymography (A,B,C). In experiments marked with the symbol +, cells were preincubated with 10 μM SB 203580, a specific inhibitor of p38 kinase, 45 minutes at 37°C before the contact with ligands (B,C).