T cells produce MMP-9 on stimulation with gp120 or pseudotyped virus in a CD4-independent manner.

Analysis of MMP-9 secretion by CD4⁻/CXCR4⁺Jurkat cells (A) and human TH1 clone CB.X.212 (B) in response to stimulation by different ligands, gp120 HXB2, SDF-1α, or IL1-α at the indicated concentration or gp120 HXB2 (10 μg/mL) inactivated AT2 pseudotyped virus containing a CD4-independent mutated envelope (20 ng p24), TNF-α (20 ng/mL), SDF-1α (100 nM), or AT2. In these experiments, cells were pretreated or not with 10 μM SB 203580 (marked with the symbol +) prior to exposure with the different ligands for 16 hours at 37°C. Supernatants were collected, lyophilized, and assayed for their gelatinase content by zymography.