Lack of effect of the ERK kinase inhibitor PD98059 on thrombin-induced IL-8 and MCP-1 production.

HUVECs were cultured with or without various concentrations of the specific MEK-1 inhibitor PD98059 or 2 μM of the p38 inhibitor SB203580 and then were stimulated with 8 U/mL thrombin for 24 hours before chemokine measurements in the supernatants, IL-8 (A) and MCP-1 (B); *P < .005, **P < .01 compared with thrombin alone (n = 4).