Effects of SB203580 and PD98059 on TRAP-induced IL-8 and MCP-1 endothelial production.

To determine whether the specific thrombin PAR-1 receptor was linked to the p38 or p42/44 MAPK pathway, HUVECs were stimulated with 100 μM TRAP in the presence or absence of SB203580 (2 μM) and PD98059 (10 μM). After 24 hours in culture, the supernatants were collected and assayed for chemokines, IL-8 (A) and MCP-1 (B); *P < .0001, **P < .001 compared with TRAP alone (n = 3).