Effects of SB203580 on thrombin-induced IL-8 and MCP-1 steady-state mRNA levels.

PCR products of RNA prepared from HUVECs stimulated with thrombin (8 U/mL) for 6 hours in the absence or presence of 2 μM SB203580 were deposited on a 2% agarose gel. SB203580 decreased IL-8 mRNA steady-state levels by almost 50% and MCP-1 mRNA by 36% (1 experiment representative of 3).