Effects of SB203580 on the chemotactic activity of thrombin-stimulated HUVEC supernatants on polymorphonuclear and THP-1 cells.

HUVECs were stimulated with 8 U/mL thrombin for 24 hours in the absence or presence of 2 μM SB203580. The supernatants were then collected, diluted, and assayed for their chemotactic activity on⁵¹Cr-labeled neutrophils (A) and monocytic THP-1 cells (B) through Transwell chamber devices. Thrombin-stimulated HUVEC supernatant chemotactic activity was considered 100% (*P < .01, **P < .005 compared with respective controls, n = 3).