Anemia and reduced CFCs inPbx1^−/− embryos.

(A) Hematocrits (Hcts) and percentage of nucleated red blood cells (nRBCs) in E15 embryos; number of embryos were 6 (+/+), 9 (+/−), and 5 (−/−). (B) Fetal liver cell counts at E12.5, E14.5, and E15.5 (15 or more FLs used for each data point).Pbx1⁺ shows combined wt and heterozygous embryos. P < .05 for differences at E14.5 and E15.5. (C) Colonies of each type scored by morphological criteria per liver on the basis of FL total cell counts. Asterisk denotes P < .05 for Pbx1^−/−compared with wt. Pbx1⁺ denotes data pooled from wt and heterozygous cells. Data are the the means and SDs obtained from 16 (−/−), 15 (+/+), and 27 (+/−) FLs of each genotype, respectively.