Fig. 6.
Fig. 6. Reduced frequency and altered lineage potentials of common myeloid progenitors. / (A) Flow cytometric analysis of hematopoietic progenitor populations in wt (left) and Pbx1−/− (right) FL at E14.5. Percentages for each subset are calculated from the lin−c-Kit+Sca-1− population. (B) Total numbers of progenitors of each type per FL are expressed as percentage of wild type. (C) Cumulative colony types are shown for purified progenitors that were plated as single cells in methylcellulose cultures. CMPs (FcγRlo/CD34+) gave rise to macrophage (Mac), granulocyte/macrophage (GM), erythroid (E), erythroid/megakaryocytic (E + Me), and mixed colonies. MEPs (FcγRlo/CD34−) produced only E, Me, and E + Me colonies, but fewer than 25% ofPbx1−/− MEPs produced colonies. Pbx1+ denotes data pooled from wt and heterozygous cells.

Reduced frequency and altered lineage potentials of common myeloid progenitors.

(A) Flow cytometric analysis of hematopoietic progenitor populations in wt (left) and Pbx1−/−(right) FL at E14.5. Percentages for each subset are calculated from the lin−c-Kit+Sca-1− population. (B) Total numbers of progenitors of each type per FL are expressed as percentage of wild type. (C) Cumulative colony types are shown for purified progenitors that were plated as single cells in methylcellulose cultures. CMPs (FcγRlo/CD34+) gave rise to macrophage (Mac), granulocyte/macrophage (GM), erythroid (E), erythroid/megakaryocytic (E + Me), and mixed colonies. MEPs (FcγRlo/CD34−) produced only E, Me, and E + Me colonies, but fewer than 25% ofPbx1−/−MEPs produced colonies. Pbx1+ denotes data pooled from wt and heterozygous cells.

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