Fig. 3.
Schematic representation of sequential transplant experiments.
A total of 40 × 106 male BALB/c bone marrow cells were injected intravenously into 0- or 1 Gy–treated female BALB/c recipients. At 8 weeks, hosts were killed and engraftment evaluated by FISH for Y chromosomes. A secondary transplant was performed using 20 × 106 chimeric bone marrow cells into 10 Gy–treated female BALB/c. In the same way, a tertiary transplant was performed.
