Fig. 7.
PS-exposing cells do not show sustained high intracellular Ca++.
(A) The extent of AV fluorescence is compared with Fluo3 fluorescence in sickle RBCs. The cells were loaded with Fluo3-AM for 1 hour and subsequently labeled with biotin-AV and PE-SA (data are shown for 1 experiment that was typical of 5 similar experiments). (B) The distribution of the same sickle cells as in panel A is plotted as a function of Fluo3 fluorescence (curve a) and compared with the distribution of cells loaded in vitro with Ca++ by incubation with the calcium ionophore A23187 in presence of 2 mM CaCl2 (curve b).
