The t(2;19)(p23;p13.1) fuses

TPM4 to ALK. (A) Photograph of ethidium bromide–stained gel of RT-PCR products. An approximately 300-bp product was amplified with primers for TPM3 andALK from patient peripheral blood (PB) and bone marrow (BM), whereas no product was amplified from control cell lines K562 and RCH-ACV, or from a water-negative control. The results of control RT-PCR analyses for a portion of the ABL complementary DNA, verifying the integrity of isolated RNA, are shown at right. (B) Nucleotide sequence of amplified portion ofTPM4-ALK complementary DNA obtained usingTPM4-specific and ALK primers. The initiation codon in TPM4 is underlined and arrows denote the point of fusion between TPM4 (nucleotides 1 > 670) andALK sequences (671 > 966).