![Fig. 4. Expression of gp130 and gp80 in HD cell lines. / (A) Immunoblot of gp130 expression in HD cell lines using the monoclonal antibody B-T12. Proteins isolated from 2 × 105 cells/lane were separated on a discontinuous SDS-PAGE with a 7.5% acrylamide resolving gel and electroblotted onto nitrocellulose filters. Specifically bound α-gp130 antibodies were detected using peroxidase-coupled secondary antibodies and ECL (unspecific bands [#]). gp130* is a faster migrating variant of gp130 detected in KMH2. The gray bar indicates the different migrations of gp130, which are probably due to differential glycosylation. (B) Expression of gp130 and gp80 on the surface of HD-derived cell lines visualized by FACS using the monoclonal antibody B-S12 for gp130 and B-R6 for gp80. Cells were incubated with unconjugated monoclonal antibodies and stained with goat-α-mouse-PE (GAM-PE) antibodies. Filled histograms indicate staining of gp130 or gp80; open histograms show staining with isotype control antibody.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/3/10.1182_blood.v98.3.762/5/h81511331004.jpeg?Expires=1735583119&Signature=aFRcvIUtV0os-Z1GN4tTTzeajYB-SzAXs3dO2~-01fWjIGUaeF4p0jsh3u82O3-kPAN5s4dITts4lbgLyOMM~vRjNiOXhKiBc8lzaUXwr2Lh7C8Ybfmllq9SPvh6xDZfBEihV4ABj72r6wqlbeiqkVFOncu8Os~U7GjDbbBUFP2Eab4YQ3nEO0bmyZcXcWUxONV6cu7vEK09pT8oCAbfjFCyEz-s7KROLj9xdaXZmsHsoQ3uVdis9uWAqL5~nozuE-jIRGBI7iqglyT-urzjSlWLhUV6GkQOrbVCctNQ3FU0GgalQc83xSS28GqKwkjLwokaNqtiZO7R-F3q9OZpBg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of gp130 and gp80 in HD cell lines.
(A) Immunoblot of gp130 expression in HD cell lines using the monoclonal antibody B-T12. Proteins isolated from 2 × 105 cells/lane were separated on a discontinuous SDS-PAGE with a 7.5% acrylamide resolving gel and electroblotted onto nitrocellulose filters. Specifically bound α-gp130 antibodies were detected using peroxidase-coupled secondary antibodies and ECL (unspecific bands [#]). gp130* is a faster migrating variant of gp130 detected in KMH2. The gray bar indicates the different migrations of gp130, which are probably due to differential glycosylation. (B) Expression of gp130 and gp80 on the surface of HD-derived cell lines visualized by FACS using the monoclonal antibody B-S12 for gp130 and B-R6 for gp80. Cells were incubated with unconjugated monoclonal antibodies and stained with goat-α-mouse-PE (GAM-PE) antibodies. Filled histograms indicate staining of gp130 or gp80; open histograms show staining with isotype control antibody.
