Autoradiograph of plasma vWF:Ag multimers of the affected members of families 1, 2, and 3.

Plasma samples electrophoresed in a 1.8% SDS-agarose gel and visualized by sodium iodide 125–labeled anti-vWF:Ag monoclonal antibody showing the abnormal multimer band patterns in F1-P1, F1-P2, F1-P3, F2-P1, and F3-P1 in lanes 2, 3, 4, 6, and 7, respectively. A type IID phenotype international reference plasma11 19 is shown in lane 8, and 2 normal plasmas are shown in lanes 1 and 5. The direction of electrophoresis is from top to bottom. The arrow in lane 7 indicates the faster migrating central band 1 in F3-P1. Differences in the minor satellite bands between normal and IID plasma (2 bands in normal and 3 bands in IID plasma) are shown in lanes 2 and 5, respectively.