Relative fluorescence intensity of stained and unstained cells (autofluorescence) through green, red, and blue channels.

(A-C) The mean RFIC and the 95% data intervals (± 1.96 SD) of fetal erythroblasts stained with antiglobin antibodies conjugated with FITC, PE, and AMCA, respectively. (D) The autofluorescence of unstained fetal erythroblasts when viewed through the green, red, and blue filters. There is overlap between the brighter autofluoresecent and weakly stained cells in the green and red channels but not in the blue.