CDR3 size distribution analysis of BV14 or BV16 cDNA from PBLs in patient 22.

The cDNA amplified using primers specific to BV16 subfamilies coupled with a fluorescent Cβ primer was analyzed for size with an automated DNA sequencer and Genescan software. Fluorescence intensity (y axis) is plotted against CDR3 size (x axis). (A) BV14 indicating normal pattern. (B) BV16 indicating abnormal pattern with high-fluorescence intensity. (C) Abnormal pattern after 10 times dilution of panel B indicating monoclonal proliferation of T cells.