Flow cytometry analysis of TR expression.

(A) Flow cytometry analysis of TR on HMCLs and LCLs. Cells were stained with an FITC-conjugated anti-TR MoAb (filled histograms) or an isotype-matched control antibody (open histograms). (B) RNA expression of TR in the same set of cell lines as assessed by the Atlas array (log base 2 transformed and median centered). (C) Flow cytometry analysis of TR in 4 primary myeloma cell samples (MM3-6), in PPCs from one patient with RP, and in TPC1 and 2. TR expression was assessed by 2-color flow cytometry using a PE anti-TR and an FITC-conjugated MI15 antisyndecan-1 MoAb. Filled histograms show the intensity of the TR staining in the plasma cell population identified as the syndecan-1⁺ cell population within the region of viable mononuclear cells defined by forward/side scatter profile. Open histograms show the staining by a PE-conjugated isotype-matched control MoAb in the plasma cell population.