Rates of dNMPS hydrolysis by ecto-5′-NT of HUVECs.

Ecto-enzymatic degradation of dNMPS by HUVECs. The catabolism was determined by reversed- phase HPLC of subsamples taken at 0, 24, 48, 72, and 96 hours after addition of dAMPS, dGMPS or TMPS (200 μM) to adhered cells (40 000 per well per milliliter). The ordinate axis shows the concentration of unchanged substrate. Data points represent means ± SDs from 3 experiments.