The influence of dNMP, dNMPS alone, and dNMPS in the presence of α,β-methylene–ADP or α,β-methylene–ATP on HUVEC growth.

HUVEC viability (expressed as the percentage of control cell counts) was determined with MTT assay 72 hours after addition of 100 μM dNMP, dNMPS, or dNMPS following 2-hour incubation with 100 μM α,β-methylene–ADP (indicated as dNMPS*) or with 100 μM α,β-methylene–ATP (indicated as dNMPS**). Cells (5000 per 200 μL) were incubated in RPMI 1640 medium supplemented with 20% FBS. Data points represent means ± SDs from at least 3 experiments.