American Society of Hematology

Fig. 4.

$Fig. 4. Nfv inhibits apoptosis induced by PTPC opening. / (A) Jurkat T cells were pretreated with MeOH (♦) or 7 μM Nfv (■) for 1 hour before overnight stimulation with the indicated doses of Vpr. Apoptosis was assessed by annexin-V and propidium iodide. Data are representative of 3 independent experiments. (B) Mitochondrial Δψm was assessed using DiOC6. Data are representative of 3 independent experiments. ♦, Vpr; ■, Vpr + Nfv. (C) Jurkat cells were treated with MeOH or 7 μM Nfv for 1 hour before overnight stimulation with 2.5 μM bovine serum albumin (BSA) or 2.5 μM Vpr and immunoblotted for cytochrome-c release (upper) and pro–caspase-8 cleavage (lower) in the cytosolic fraction. (D) Immunoblot of Hsp 70 in pellet and cytosolic fractions. (E) Isolated mitochondria were treated overnight with methanol (▪), 7 μM Nfv (░), or 50 μM BA (▤) before being stimulated with 2.5 μM Vpr or 5 mM ATR. Loss of Δψm was assessed using DiOC6.$

Nfv inhibits apoptosis induced by PTPC opening.

(A) Jurkat T cells were pretreated with MeOH (♦) or 7 μM Nfv (■) for 1 hour before overnight stimulation with the indicated doses of Vpr. Apoptosis was assessed by annexin-V and propidium iodide. Data are representative of 3 independent experiments. (B) Mitochondrial Δψ_m was assessed using DiOC₆. Data are representative of 3 independent experiments. ♦, Vpr; ■, Vpr + Nfv. (C) Jurkat cells were treated with MeOH or 7 μM Nfv for 1 hour before overnight stimulation with 2.5 μM bovine serum albumin (BSA) or 2.5 μM Vpr and immunoblotted for cytochrome-c release (upper) and pro–caspase-8 cleavage (lower) in the cytosolic fraction. (D) Immunoblot of Hsp 70 in pellet and cytosolic fractions. (E) Isolated mitochondria were treated overnight with methanol (▪), 7 μM Nfv (░), or 50 μM BA (▤) before being stimulated with 2.5 μM Vpr or 5 mM ATR. Loss of Δψ_m was assessed using DiOC₆.

This Feature Is Available To Subscribers Only