Fig. 2.
Fig. 2. The p12I protein increases STAT5b DNA binding and does not interfere with STAT5b degradation. / (A) EMSA of lysates from COS-7 cells. Top panel shows control for the amount of IL-2R β expression on Western blot analysis, analysis of p12I expression by Western blotting, and p12Iand IL-2R β chain coprecipitation. (B) Transfected cells were pulsed with 10 nM IL-2 for 20 minutes, washed, incubated in medium without IL-2 for the indicated times, lysed, and processed for EMSA as in Figure 2A. (C) Graph shows quantitation of the data in Figure 2B, obtained by using a PhosphorImager (Molecular Dynamics, Sunnyvale, CA). C indicates control; IP, immunoprecipitation; WB, Western blot.

The p12I protein increases STAT5b DNA binding and does not interfere with STAT5b degradation.

(A) EMSA of lysates from COS-7 cells. Top panel shows control for the amount of IL-2R β expression on Western blot analysis, analysis of p12I expression by Western blotting, and p12Iand IL-2R β chain coprecipitation. (B) Transfected cells were pulsed with 10 nM IL-2 for 20 minutes, washed, incubated in medium without IL-2 for the indicated times, lysed, and processed for EMSA as in Figure 2A. (C) Graph shows quantitation of the data in Figure 2B, obtained by using a PhosphorImager (Molecular Dynamics, Sunnyvale, CA). C indicates control; IP, immunoprecipitation; WB, Western blot.

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