Flow cytometry analysis of the binding of mAbs to WT and GPV-deficient platelets.

Antibodies against mouse GPV (1C2), GPVI (JAQ1), and α2β1 integrin (HMα2) were incubated with washed platelets from GPV null (−/−) and WT (+/+) mice, and their binding was quantified by flow cytometry. Analysis of the FL1 histograms confirmed a lack of GPV on GPV null platelets, whereas GPVI and the α2β1 integrin were expressed at similar levels on WT and GPV-deficient platelets.