Inhibition of ex vivo collagen-induced aggregation of rat platelets following injection of V.3.

Wistar rats were given a bolus intravenous injection (1 mg/kg) of the mAb V.3, which cross-reacts with rat GPV, or the mAb V.1, which does not recognize rat GPV. Blood was drawn 20 minutes later for aggregation tests in citrated PRP (A, B) and platelet counts (C). Treatment with V.3 resulted in decreased amplitude of aggregation (A) and increased lag phase (B) in response to collagen compared to treatment with V.1, but it had no effect on the ADP response (A). There was no significant change in platelet count after V.3 or V.1 injection (C). Results are the mean values ± SEMs for 3 animals in each group.