Fig. 1.
Telomerase activity in CD8
+ T clones transduced with a GFP control vector or with an hTERT/GFP vector. The 104 transduced cells after cloning step II (lanes 1-4) and V (lanes 5-8) were analyzed for telomerase expression by the TRAP assay.23 The 4 subclones are depicted as follows: T11.9 = lanes 1 and 5, T11.14 = lanes 2 and 6, G11.8 = lanes 3 and 7, G11.1 = lanes 4 and 8. T11.9 and T11.14 are clones transduced with hTERT construct, whereas G11.8 and G11.1 are clones transduced with the GFP control vector. Positive control extracts obtained from HeLa cell line and negative controls using RNAse-treated extracts were used in each experiment (data not shown).
