Fig. 1.
Immunophenotypic analysis of isotype proteins on HCs.
HCs were identified in the R1 region as CD11c+ (FL2) and CD19+ (FL3) and gated in R2. Cells in R1 and R2 were then analyzed for the surface expression of IgM, IgD, IgG, and IgA with FITC(FL1)-conjugated F(ab′)2 monoclonal antibody specific to each class. This example represents patient 163. The unfilled curves in the histogram plots represent the isotype control.