Coassociation of calmodulin and GPIb-IX-V in platelets.

Triton X-100 soluble and actin cytoskeletal (CYSK) fractions isolated from platelets either unstimulated or stimulated with thrombin for 5 minutes were immunoprecipitated (IP) by means of GPIb antibodies (A) or GPV antibodies (B) compared with nonimmune sera (NonI), and immunoblotted by means of antibodies to calmodulin. Blots were visualized by means of a peroxidase-coupled second antibody and the ECL reagent. Results are typical of 3 separate experiments.