Phenotype of monocyte-derived DCs.

Monocytes from allergic patients or from healthy donors were cultured in the presence of GM-CSF and IL-4 for 7 days. The cells were then collected and analyzed by flow cytometry for CD1a, CD11c, HLA-DR, and CD83 expression (filled histograms). Open histograms represent the reactivity of fluorochrome-matched isotype control mAbs.