Effect of Der p 1 on CD86 and CD80 expression on DCs.

Monocyte-derived DCs from house dust mite–sensitive patients (left panels), from grass pollen–sensitive patients (middle panels), and from healthy donors (right panels) were exposed or not to Der p 1, ProDer p 1, or LPS for 24 hours. DCs were then incubated with PE-conjugated anti-CD86 mAbs or with FITC-conjugated anti-CD80 mAbs, electronically gated, and analyzed for CD86 and CD80 expression (filled histograms). Open histograms represent reactivities of fluorochrome-matched isotype control mAbs. One representative experiment of 7 is shown.