Identification of the murine
DUB-2 and DUB-2A genes by Southern blot analysis. (A) Schematic representation of theDUB-2 gene. The indicated primers were used to amplify theDUB-2 and DUB-2A genes from murine BAC clones by PCR. The indicated 800-bp probe was used for Southern blot analysis in panel B. (B) Genomic DNA from the indicated murine cell lines or BAC clones was restriction-digested with EcoRV, electrophoresed, blotted to nitrocellulose, and probed with the 32P-labeled probe. Lanes 1 to 7 contain EcoRV-digested genomic DNA from the indicated murine cell lines. Ba/F3 and 32D cells were derived from Balb/c mice. Cytotoxic T-lymphocyte line (CTLL) cells were derived from C57BL mice. HCD57 cells were derived from National Institutes of Health (NIH) Swiss mice. B16 cells were derived from B16 mice. FVB cells were derived from FVB mice. ES cells were derived from 129SV mice. Lanes 8 and 10 contain digested DNA from either a DUB-2 genomic clone or a DUB-2A (BAC1) genomic clone, respectively. The band corresponding to the DUB-2 and DUB-2A genes is indicated.
