identification and functional analysis of the cytokine-inducible enhancer of the

DUB-2 gene. (A) Nucleotide sequence of theDUB-2A promoter. A putative TATA box (position −104) is indicated with a short underline. A 100-bp enhancer element is indicated with a long underline. In addition, a purine-rich microsatellite repeat is found between positions −560 and −385. (B) Comparison of the minimal enhancer regions of DUB-1 andDUB-2A. These enhancer regions contain an ETS protein consensus sequence, 2 AP1 sites, and 3′GATA site. A CBF site, a 5′GATA site, and a TG protein–binding site are found only in DUB-1,as indicated. (C) Luciferase activity was assayed in Ba/F3 cells transfected with the indicated constructs. The cells were starved and restimulated with no growth factor (■) or 10 pM IL-3 (▪). Luciferase assays were performed after 8 hours.