Histologic examination reveals zonal hepatic microvesicular steatosis inFancc^−/−Sod1^−/−mice.

Histology of Fancc^−/−Sod1^−/−and control livers (magnification × 400). Sections ofFancc^−/−(A,E),Sod1^−/−(B,F),Fancc^+/−Sod1^+/−(C,G),and Fancc^−/−Sod1^−/−(D,H) livers were stained with Masson trichrome (A-D) and oil red-O (E-H). WhereasFancc^−/−, Sod1^−/−, andFancc^+/−Sod1^+/−cells show normal morphology, Fancc^−/−Sod1^−/−hepatocytes demonstrate a zone 3 abnormality characterized by abundant cytoplasmic vacuolation. Staining with oil red-O demonstrates prominent microvesicular zone 3 lipid accumulation without nuclear displacement in Fancc^−/−Sod1^−/−mice. Small amounts of lipid droplets are present inSod1^−/−mice.