Localization of P-selectin, PMNs, and platelets 6 hours after LPS injection.

(A) P-selectin was expressed on sinusoidal endothelia of the liver (arrows), not the splenic sinusoids (B), nor the pulmonary capillaries (C), although some trapped platelets (B, blue spots) and large vessels (C, arrows) were labeled. (D) PMNs were labeled with Ab HIS48 (brown) in the hepatic sinusoids, splenic sinusoids (E), and pulmonary capillaries (F). (G) Platelets in hepatic sinusoids were labeled by MoAb CD61 (arrows). Scale bars, panels A-F, 20 μm; panel G, 10 μm.