Number of PMNs after various treatments.
(A) Number of PMNs reached maximal levels at 1 hour in spleen and lungs, 6 hours in the liver after LPS injection. (B) Number of PMNs was reduced in the liver after treatment with anti–P-selectin Ab or LMWH or fucoidin; significant increases were observed in spleen and lungs, compared with LPS treatment alone. (C) Percent of PMNs in peripheral blood increased and combined treatment increased more and peaked 6 hours after LPS injection. (D) About 2-fold higher total leukocyte count with anti–P-selectin Ab or LMWH or fucoidin than control was observed in peripheral blood. (E) Percent of circulating TUNEL-positive cells showed no significance between saline and LPS treatment; however, a significant increase was observed 6 hours after GdCl3injection.
