Apoptotic cells after various treatments.

A number of TUNEL-positive cells (blue spots: spleen [A], lungs [C]) and annexin V-positive cells (spleen [B], lungs [D]) were labeled. (E) The number of TUNEL-positive cells in the liver peaked 6 hours after LPS; however, a significant increase was observed in spleen and lungs only after combined treatment with LPS and GdCl₃. (F) A number of annexin V-positive cells were observed in the liver 6 hours after LPS. But significant increases were found in spleen and lungs after GdCl₃ treatment. (G) The number of phagocytosed cells was reduced, and significant reduction was observed in the liver 6 hours after combined treatment with annexin V, compared with LPS treatment alone. *P < .05 versus LPS treatment only. Scale bar, 20 μm.