N-WASP is expressed in activated murine B lymphocytes.

Detergent lysates of B cells, stimulated with LPS or LPS plus IL-4 for 46 to 50 hours, were separated using SDS-PAGE, blotted, and exposed to antibodies against WASP or N-WASP. (A) Anti-WASP antibodies recognized WASP in WASP⁺ B cells and in an unknown, higher molecular species (asterisk) in both WASP⁺ and WASP⁻ B cells. (B) Anti–N-WASP antibody recognized a band consisting of 2, perhaps 3, species that most likely represent different phosphorylation states of N-WASP. These data are representative of 3 similar experiments.