Zebrafish mpoexpression.

In situ hybridization using a digoxigenin-labeled RNA probe to detect zebrafish mpo in embryos at 22 hpf (A) and at 3 to 4 dpf (B-C) and in a paraffin-embedded adult kidney section (D). Sections counterstained with methyl green (Vector, Burlingame, CA). Black arrowheads indicate mpo expression in the posterior ICM (A-B). Black arrows indicate mpo expression in neutrophils, note the bi-lobed nuclei of positive cells. Lateral views, dorsal upward, anterior to the left (A-C); 150 × (D).