Rapid induction of apoptosis in cultures containing IFN-α and LPS.

On day 0, 1000 U/mL IFN-α ± 1 ng/mL LPS was added to monocyte cultures supplemented with GM-CSF plus IL-4. Annexin V positivity was quantified on days 2, 4, and 6. ■, control; ░, IFN-α; ▪, IFN-α plus LPS. Shown are the data obtained with monocytes from 3 different donors (mean ± SD).